Detection and Characterization of Sorbitol Dehydrogenase from Apple Callus Tissue
نویسندگان
چکیده
منابع مشابه
Detection and characterization of sorbitol dehydrogenase from apple callus tissue.
Sorbitol dehydrogenase (l-iditol:NAD(+) oxidoreductase, EC 1.1.1.14) has been detected and characterized from apple (Malus domestica cv. Granny Smith) mesocarp tissue cultures. The enzyme oxidized sorbitol, xylitol, l-arabitol, ribitol, and l-threitol in the presence of NAD. NADP could not replace NAD. Mannitol was slightly oxidized (8% of sorbitol). Other polyols that did not serve as substrat...
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Sorbitol, the primary photosynthate and translocated carbohydrate in apple (Malusxdomestica Borkh.), is converted to fructose by sorbitol dehydrogenase (SDH; EC 1.1.1.14) which is active in apple fruit throughout development. In the apple genome, nine SDH genes have been isolated and their sequences characterized, but their individual expression patterns during apple fruit set and development h...
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Sorbitol plays a key role in the translocation of photosynthate in the Rosaceae family, including apple (Malus domestica), pear, and stone fruits (4, 7). NADP-S6PDH2 plays the most important role in biosynthesis of sorbitol in apple because its activity increases before accumulation of sorbitol in seedlings (8), and high activity is maintained in leaves (10). This enzyme reduces G6P to S6P usin...
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متن کاملUbiquitous distribution and different subcellular localization of sorbitol dehydrogenase in fruit and leaf of apple
NAD(+)-dependent sorbitol dehydrogenase (NAD-SDH, EC 1.1.1.14), a key enzyme in sorbitol metabolism, plays an important role in regulating sink strength and determining the quality of apple fruit. Understanding the tissue and subcellular localization of NAD-SDH is helpful for understanding sorbitol metabolism in the apple. In this study, two NAD-SDH cDNA sequences were isolated from apple fruit...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1979
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.64.1.69